Efectele aflatoxinelor asupra oamenilor și animalelor - metode analitice de detecție

Abstract

Aflatoxins contaminations are true threat because of their negative effects for animal and human health. Aflatoxicosis, resulting from the ingestion of aflatoxin-contaminated food or feed, primarily affects the liver. Vomiting, abdominal pain, pulmonary edema, convulsions, coma, and death characterize these clinical manifestations. Aflatoxin B1 is a potent liver carcinogen. Types of cancers described in research animals include hepato-cellular, colon, and kidney carcinoma (rats), cholangiocellular cancer (hamsters), lung adenomas (mice), and osteogenic sarcoma, adenocarcinoma of the gall bladder and carcinoma of the pancreas (monkeys). It has been studied the possibilities to detect aflatoxins using different analytical methods: ELISA, chromatography (thin layer chromatography and HPLC), and amperometric detection; the amperometric detection was performed using free and immobilised acetyl-cholinesterase (Electric eel or mutant Drosophila melanogaster).