Markeri de activare plachetara în flow-citometrie la pacienţii cu sindrom antifosfolipidic primar

Abstract

The present paper describes a flow cytometric method for the assay of platelet activation and provides information important in identifying and differentiating thrombogenic states in patients with primary antiphospholipid syndrome (SAFP). Objectives: to evaluate the significance of platelet activation markers for the evolution of venous and arterial recurrent thrombosis in patients (pts). with SAFP. Methods: 14 pts. with SAFP diagnosed according to revised Sapporo classification for APS criteria, mean age 47.87(18-74) years, 7 women and 7 men were followed 24 months for the evolution with recurrent thrombosis. The expression of platelet activation markers CD62P (Pselectin) and CD40L were assessed using a standardized flow cytometric method (Becton Dickinson) at baseline and after 12 months. Statistics: The results are shown as mean ± SD. Paired t-tests were used for statistical comparisons. Results: No significant correlation was observed between the expression of platelet activation markers CD62P (Pselectin) and CD40L in patients with a history of recurrent thrombosis and SAFP patients without recurrent thrombosis. One single patient from six pts. had an acute thrombotic event during follow-up expressed markers of platelet activation. Conclusions: Platelet activation markers in flow cytometry did not correlate with recurrent thrombosis and acute thrombosis in pts. with SAFP.