Optimization of Ultrasonic-Assisted Extraction of Polysaccharides from Quince Branch by Response Surface Methodology (RSM)

Abstract

The objective of this study is to investigate the impact of ultrasonic waves on the extraction yield of Quince branch crude polysaccharides (QBCP). To optimize the effects of the ultrasonic-assisted extraction (UAE) processing parameters on the extraction yield of QBCP, a response surface methodology (RSM) was employed. Four independent variables were investigated: extraction temperature of 50◦C to 90◦C, extraction time of 20 to 60 minutes, pH of 3 to 90, and solid to volume ratio of 1 to 13%. The obtained experimental data were fitted to a second-order polynomial equation using multiple regression analysis and also analyzed by appropriate statistical methods (ANOVA). The response surface methodology (RSM) showed that the significant quadratic regression equation with high R2 (=0.9872) was successfully fitted for extraction yield of QBCP as a function of independent variables. The optimum conditions for the extraction procedure of crude polysaccharides from the quince branch were identified as follows: extraction temperature of 80◦C, extraction time of 30 minutes, pH equal to 4.51, and solid to volume ratio of 4.3%. Under these conditions, the experimental yield was (9.03 ± 0.98%), which is in close agreement with the value predicted by the model. In general, the extracted polysaccharide has various usages in the pharmacy and food industries.