DNA Isolation and Amplification in Oak Species (Quercus spp.)

Abstract

Two DNA isolating protocols were tested and compared at species from the Quercus genus for their ability to produce DNA in good yield and purity. Both procedures were adapted to the conditions of our laboratory. The DNA resulting from isolation was amplified through a polymerase chain reaction. The best results were obtained by using strong dilutions (1:100) which very likely reduce the number of inhibitors such as tannins, phenols, and polysaccharides. At this dilution, the success of DNA amplification was almost 95% for long fragments from chloroplast DNA and almost 100% for short fragments from nuclear DNA such as SSRs (simple sequence repeats).