Antioxidant Activity of Acetilcysteine Proved in Vitro

Abstract

Peroxydases are to be found in biological liquids and have the propriety of catalyzing the discomposure of oxygenated water, formed at these levels. The first part of the present paper presents an analysis of the latest data in the specialty literature regarding the exposure of the lung to oxidative stress, as the effect of free radicals upon the induction of pulmonary pathology is already known. The second part presents a study based on kinetic analysis. The study proves that the acetylcisteine is an activator upon the 3,3' -diaminobenzidine- H2O2-peroxidase system. The imunoenzymatic technique called ELISA enables some qualitative and quantitative determinations, based on the action of the enzyme upon a specific substratum that changes its structure and the possibility of absorbing the radiation in the ultraviolet (UV) spectrum or visible spectrum (VIS). The authors have characterized the kinetic of enzymatic reactions; they have investigated the dependence of extinction according to time and the dependence of velocity according to concentration. The team has also determined the percentage of enzymatic activity, so as to highlight the extent to which the acetylcysteine has an activating character in the given system. Following the experiments, the team of researchers has shown the activating influence of acetylcysteine upon the 3,3' -diaminobenzidine-H2O2-peroxidase system.