Competitive Elisa for Aflatoxins and Ochratoxins Detection

Abstract

Mycotoxins have attracted worldwide attention because of their  impact on humans and animals health.  The most frequent toxigenic fungi in Europe are Aspergillus, Penicillium and  Fusarium species. They produce aflatoxin B1 transformed into aflatoxin M1  found in the milk, as well as Ochratoxins and Zearalenone. These mycotoxins  are under continuous survey in Europe, but the regulatory aspects still need  to be set up and/or harmonised at European level.  Direct competitive ELISA was used for aflatoxin M1 and ochratoxin A  detection from reference and real samples (milk, coffee). Calibration curves  were plotted and limits of quantification were calculated. A recovery degree  of 98.66% was obtained for spiked sample (coffee).