Laboratory Technique for Diagnosis of Autoimmune Diseases

Abstract

The Fluorescence Immunoassay and Enzyme-linked immunosorbent- assay (ELISA) can be used as an aid in the diagnosis of certain autoimmune diseases, being extremely important in medical practice. The study was conducted on a total of 75 patients registered between 11.11.2011-16.02.2012, in the Clinical Emergency Hospital of Brasov. The aims of this paper are to highlight the importance of titration in patients with antinuclear antibodies presents and the importance of ELISA technique for the diagnosis of autoimmune diseases. Fluorescence can be used as a screening method for determination of antinuclear antibodies. A positive titer accompanied by the appearance pattern orients us on the correct and fast diagnosis. Both fluorescence and ELISA are important for diagnosing and monitoring autoimmune diseases.