The Inhibitory Effect of the Product Dihydroxyacetone on an Aerobe Bacterium in a Semi-Continuous Two-Stage Repeated-Fed-Batch Process

Abstract

The influence of the product inhibition by dihydroxyacetone (DHA) on Gluconobacter oxydans for a novel semi-continuous two-stage repeated-fed-batch process was examined quantitatively. It was shown that the culture was able to grow up to a DHA concentration of 80 kg/m3 without any influence of product inhibition. The reachable maximum final DHA concentration was as high as 220 kg/m3. The lag phases for growth increased exponentially with increasing DHA threshold values of the first reactor stage.