Trypsin Immobilisation on Gold Chips by Self-Assembling Process

Abstract

The aim of this paper is to immobilize trypsin on the solid surface and maintain the activity and stability of the immobilized protein. The covalent immobilization of trypsin onto 11-mercaptoundecanoic acid (MUA) modified gold chip surfaces was done due to the amide bond formation with the N-hydroxysuccinimide ester intermediate. The trypsin immobilization onto a solid surface is a challenging goal in biotechnology, molecular electronics, and biosensors.